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Stimulation of collagen and elastin synthesis pathways

Reports of gene-expression changes affecting thousands of genes at certain doses and exposure times are unusual in the peptide literature, and the interpretation has been an area where the more enthusiastic readings (the compound has broad regulatory activity across many cellular processes) and the more cautious readings (broad gene-expression effects can reflect secondary consequences and may not all be specific or mechanism-based) coexist in the discourse

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M199 medium supplemented with 2% BSA, 1% ITS, 1% BDM, 1% CD lipids and 1% penicillin/streptomycin was utilized to culture the CMs